Biochip Surfaces Containing Recombinant Cell-Binding Domains of Fibronectin

Surface immobilization and characterization of the functional activity fibronectin (Fn) type-III domains are reported. The FnIII9-10 or FnIII10 containing RGD loop PHSRN synergy site were recombinantly produced covalently bound to chemically activated PEG methacrylate (MA) hydrogel coatings by microcontact printing. Such fabricated biochip surfaces 6 mm in diameter consisted 190 µm wide protein stripes separated 200 spacing. They analyzed imaging null ellipsometry, atomic force microscopy fluorescence microscopy. Also, tested human foreskin fibroblast HeLa cultures for at least 96 h, thus evaluating their suitability controlled cell adhesion proliferation. However, while equally well responsive FnIII9-10, Fn surfaces, fibroblasts displayed lower focal areas, as proliferation rates on fragment compared Fn. Nevertheless, full was confirmed immunostaining cells adherent surfaces. observed interaction differences that either type surface composition-dependent demonstrate potential use specifically engineered other ECM protein-derived architectures.